Human Retinal Microvascular Endothelial Cells

Certificate #: ACBRI 181

Introduction

Primary Human Retinal Microvascular Endothelial Cells (ACBRI 181) are initiated by elutriation of dispase dissociated normal human retina tissue.

Cell Initiation

These cells were originated using CSC Complete Serum-Free Medium (SF-4Z0-500), are available at < 12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~ 1.5 X 10⁶ cells. This vial will initiate a Passage 4 cell culture in a 75cm² flask.

These cells are available in cryopreserved vials as well as in 25cm² and 75cm² proliferating cell culture flasks.

We have other individual donor lots available. Please contact us for details.

Companion Products

Each vial or flask of cells is shipped to Customer with Bac-Off^® (antibiotic) and CultureBoost (animal derived growth factors) or CultureBoost-R (human recombinant growth factors) at no additional cost.

These cells are qualified for use with: CSC Complete Serum Free Medium (SF-4Z0-500) and CSC Complete Medium which includes 10% serum (4Z0-500); CSC Attachment Factor™ (4Z0-210); CSC Passage Reagent Group™ (4Z0-800) and CSC Cell Freezing Medium (4Z0-705).

Cell Growth Images

Cell growth images are available.

Standard Tests

TEST	RESULTS
HIV Serologic Test (donor level HIV AB EIA)	Negative
HIV PCR TEST (frozen cell pool by CLIA Licensed Clinical Lab)	Negative
Test of frozen cells for Mycoplasma spp. (ATCC method by CLIA Licensed Clinical Lab)	Negative

Miscellaneous Tests

TESTS	RESULTS
Cytoplasmic VWF / Factor VIII	> 95% positive by immunofluorescence
Cytoplasmic uptake of Di-I-Ac-LDL	> 95% positive by immunofluorescence

Citations

PMID	Title	Author	Year
21053068	Insulin and beta-adrenergic Receptors Inhibit Retinal Endothelial Cell Apoptosis Through Independent Pathways	Jena J. Steinle	2010
20421451	Pld1-dependent PKCy activation downstream to Src is essential for the development of pathologic retinal neovascularization	Gadiparthi N. Rao	2010
20110406	Inhibition of Protein Kinase c o Attenuates Blood-Retinal Barrier Breakdown in Diabetic Retinopathy	Jeong-Hun Kim	2010
20011081	Extracellular SOD and VEGF are increased in vitreous bodies from proliferative diabetic retinopathy patients	Hideaki Hara	2009
19875648	Protective Effect of Clusterin on Blood-Retinal Barrier Breakdown in Diabetic Retinopathy	Jeong-Hun Kim	2010
19857102	Calpin Inhibitor SNJ-1945 Attenuates Events Prior to Angiogenesis in Cultured Human Retinal Endothelial Cells	Hong Ma	2009
19756180	Decursin inhibits retinal neovascularization via suppression of VEGFR-2 activation	Jeong Hun Kim	2009
19494202	The Role of PGE2 Receptor EP4 in Pathologic Ocular Angiogenesis	Susan E. Yanni	2009
19349617	The 15(S)-hydroxyeicosatetraenoic acid-induced angtiogenesis requires Janus kinase 2-signal trasducer and activator of tyranscription-5B-dependent expression of interleukin-8	Gadiparthi N. Rao	2009
19232363	Soluble Ephrin-B2 Mediates Apoptosis in Retinal Neovascularization and in Endothelial Cells	Michael H. Davies	2009
18385094	Mecamylamine Suppress Basal and Nicotine-Stimulate Choroidal Neovascularization	Katsuji Kiuchi	2008
18208562	Deguelin inhibits retinal neovascularization aby down-rerulation of HIF-1alpha in oxygen-induced retinopathy	Jeong Hun Kim	2008
18436837	Dexamethasone Inhibits High Glucose-, TNF-alpha-, and IL-1beta-Induced Secretion of Inflammatory and Angiogenic Mediators from Retinal Microvascular Pericytes	Alissar Nehme	2008
18541234	Beta-adrenergic receptor stimulation modulates iNOS protein levels through p38 and ERK1/2 sinaling in human retinal endothelial cells	Jena J Steinle	2008
17962441	15(S)-HETE Production in Human Retinal Microvascular Endothelial Cells by Hypoxia: Novel Role for MEK1 in 15(S)-HETE-Induced Angiogenesis	Arun K. Bajpai	2007
17067575	Sympathetic nuerotransmission modulates expression of inflammatory markers in the rat retina	Jena J. Steinle	2007
17177965	Interferon-beta and adhesion molecules (E-selectin and s-intracellular adhesionmolecule-1) are detected in ser from patients with retinal vasculitis and are induced in retinal vascular endothelial cells by Toll-like receptor 3 signalling.	M T Lee	2007
17442832	AKAP12 Regulates Human Blood-Retinal Barrier Formation by Dounregulation of Hypoxia-Inducible Factor 1 alpha	Yoon Kyung Choi	2007
17065526	Specific Involvement of Src Family Kinase Activation in the Pathogenesis of Retinal Neovascularization	John S. Penn	2006
17023682	Tissue Factor Pathway Inhibitor-2 Is Upreegulated by Vascular Endothelial Growth Factor and Supresses Growth Factor-Induced Proliferation of Endothelial Cells	Elia J. Duh	2006
16936124	Vascular Endothelial Growth Factor Upregulates Expression of ADAMTS1 in Endothelial Cells through Protein Kinase C Signaling	Elia J Duh	2006
15037582	Susceptibility of Retinal Vascular Endothelium to Infection with Toxoplasma gondii Tachyzoites	Justine R. Smith	2004
15332016	Delivery of antiangiogenic and antioxidant drugs of ophthalmic interest through a nanoporous Inorganic filter	Kathleen E. Orosz	2004
12670949	Beta3-Adrenergic Receptors Regulate Retinal Endothelial Cell Migration and Proliferation	Jena J. Steinle	2003
12657599	Constitutive and Infalmmatory Mediator Regulated Fractalkine Experession in Human Ocular Tissues and Cultured Cells	Matthew D. Silverman	2003

ACBRI is a nonprofit research institute providing research support to biomedical and cell biology institutions/agencies/organizations. CSC is the authorized agent for distribution of ACBRI research material. Funds collected by CSC for ACBRI products are transferred to ACBRI to cover acquisition and research support costs.

IRB acquisition requirements prohibit involvement of ACBRI research material in any profit-making enterprise, and Cell Systems Corporation does not profit from handling or delivery of ACBRI products. There are specific restrictions on the employment of ACBRI cells. ACBRI cells are for research purposes only and may not be transferred out of the direct control of the Recipient Institution/agency/organization. ACBRI cells may not be genetically altered in any way without prior written permission by ACBRI. Use of ACBRI products (as documented by placement of any order for product by an authorized representative constitutes knowledge, understanding and binding acceptance of these restrictions on behalf of the recipient organization/institution.

Please read our Terms and Conditions.