Bovine Aortic Endothelial

Certificate #: CSC 2B2

Introduction

Primary Bovine Aortic Endothelial Cells (CSC 2B2) are pooled primary isolates from eight individual donor veal calf descending aortae.

These cells were originated using CSC Complete Serum-Free Medium (SF-4Z0-500), are available at < 12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~ 1.5 X 10⁶ cells. This vial will initiate a Passage 4 cell culture in a 75cm² flask.

These cells are available in both cryopreserved vials and in 75cm² proliferating culture flasks.

Each vial or flask of cells is shipped to Customer with Bac-Off^® (antibiotic) and CultureBoost (animal derived growth factors) or Cultureboost-R (human recombinant growth factors) at no additional cost.

These cells are qualified for use with all recombinant and non-recombinant versions of CSC Serum-Free and Serum-Containing (10% by volume) Complete Medium Kits; CSC Attachment Factor™ (4Z0-210); CSC Passage Reagent Group™ (4Z0-800) and CSC Cell Freezing Medium (4Z0-705).

Bovine Aortic Endothelial Cells Catalog CSC 2B2 Passage 5 40X Phase Contrast Digital Image

Primary Bovine Aortic Endothelial Cell System Proliferation Assay
9-28 CPD in vitro / Passages 3-8
CSC 2B2 Primary Bovine Aortic Endothelial Cells
4Z0-500 Complete Serum Containing Medium Kit
4Z0-800 Passage Reagent Group™ (PRG)
CSC specifies bovine serum and serum-derived components 100% traceable to cattle processed in the US at USDA-inspected facilities.

Cell Growth Images

Cell growth images are available.

Standard Tests

Test	Results
HIV PCR TEST (frozen cell pool by CLIA Licensed Clinical Lab)	Negative
Test of frozen cells for Mycoplasma spp. (ATCC method by CLIA Licensed Clinical Lab)	Negative

USDA Form 16-4 (Declaration Regarding health of the Donor Animal) May be required for some international orders. If Recipient's government requires a 16-4 Declaration for animal products, Recipient will be responsible for obtaining information from his/her Customs Officials for use by the USDA in determining whether a Form 16-4 can be issued.

**Citations**
PMID	Title	Author	Year
19038867	An Ezrin/Calpain/P13k/AMPK/eNOS s1179 Signaling Cascade Mediating VEGF-Dependent Endothelial Nitric Oxide Production	Hua Cai	2009
18787079	Activation of Hsp90-eNOS and increased NO generation attenuate respiration of hypoxia-treated endothelial cells	Tennille Presley	2008
18763312	Effect of serum concentration on adhesion of monocytic THP-1 cells onto cultured EC monolayer and EC-SMC co-culture	Li-jie Fan	2008
17220911	Statins induce S1P1 receptors and enhance endothelial nitric oxide production in response to high-density lipoproteins	J igarashi	2006
17082183	Evidence for the Pathophysiological Role of Endogenous Methylarginines in Regulation of Endothelial NO Production and Vascular Function	Arturo J. Cardounel	2006
17012319	Electron Paramagnetic Resonance Oximetry as a Quantitiative Method to Measure Cellular Respiration: A Consideration of Oxygen Diffusiion Interference	Tennille Presley	2006
16973617	Flow-activated Chloride Channels in Vascular Endothelium	Abdul I. Barakat	2006
16943246	Hydrogen peroxide induces S1P1 receptors and sensitizes vascular endothelial cells to sphingosine 1-phosphate, a platelet-derived lipid mediator	Junsuke Igarashi	2006
16895801	Early Determinants of H2O2-induced Endothelial Dysfunction	Beth M. Boulden	2006
16611730	Netrin-1 induces angiogenesis via a DCC-dependent ERK1/2-eNOS feed-forward mechanism	Andrew Nguyen	2006
15905466	Regulation of Xanthine Oxidoreductase Protein Expression by Hydrogen Peroxide and Calcium	J. Scott McNally	2005
15705727	Vascular endothelial wound closure under shear stress: role of membrane fluidity and flow-sensitive ion channels	Abdul I. Barakat	2005
15744025	Trophoblast Migration Under Flow Is Regulated by Endothelial Cells	Arlen Soghomonians	2005
15824471	Hepatocyte Growth Factor Stimulates Nitric Oxide Production through Endothelial Nitric Oside Synthase Activation by the Phosphoinositide 3-Kinase/Akt Pathway and Possibly by Mitogen-Activated Protein Kinase Kinase in Vascular Endothelial Cells	Akira Uruno	2004
15292187	Small Interfering RNA-mediated Down-regulation of Caveolin-1 Differentially Modulates Signaling Pathways in Endothelial Cells	Eva Gonzalez	2004
15256377	Effect of glycocalyx on shear-dependent albumin uptake in endothelial cells	Akinori Ueda	2004
15142848	Neutral sphingomyelinase inhibitor scyphostatin prevents and ceramide mimics mechanotransduction in vascular endothelium	Jan E. Schnitzer	2004
14761889	Differential membane potential and ion current responses to different types of shear stgress in vascular endothelial cells	Abdul I. Barakat	2004
14751809	Peroxisome Proliferator-Activated Receptor Agonists Increase Nitric Oxide Synthase Expression in Vascular Endothelial Cells	Kayoko Goya	2004
14736917	Calmodulin phosphorylation and modulation of endothelial nitric oxide synthase catalysis	Daniel M. Greif	2004
14597620	Hypoxia Potentiates Nitric Oxide-mediated Apoptosis in Endothelial Cells via Peroxynitrite-induced Activation of Mitochondria-dependent and -independent Pathways	Geoffrey a Walford	2004
14570928	Shear Stress Regulates Endothelial Nitric-oxide Synthase Promoter Activity through Nuclear Factor kB Binding	Michael E. Davis	2004
14563789	Roles of Superoxide, Peroxyitrite and Protein Kinase C in the Development of Tolerance to Nitroglycerin	G. Abou-Mohamed	2003
12963813	VEGF induces S1P1 receptors in endothelial cells: Implications for cross-talk between sphingolipid and growth factor receptors	Junsuke Igarashi	2003
12958034	Role of xanthine oxidoreductase and NAD(P)H oxidase in endothelial superoxide production in response to oscillatory shear stress	J. Scott McNally	2003
12757411	Hepatocyte growth factor activates endothelial nitric oxide synthase by Ca2+ - and phosphoinositide 3-kiase/Akt-dependent phosphorylation in aortic endothelial cells	Kennedy Makondo	2003
12692136	Interactions of Peroxynitrite, Tetrahydrobiopterin, Ascorbic Acid, and Thiols Implications for Uncoupling Endothelial Nitric-Oxcide Synthase	Sergey Dikalov	2003
12615686	Glucose-6-Phosphate Dehydrogenase Overexpression Decreases Endothelial Cel Osidant Stress and Increases Bioavailable Nitric Oxide	Jane A. Leopold	2003
12533667	Potential Involvement of the Cyclooxygenase-2 Pathwya in the Regulation of Tumor-associated Angiogenesis and Growth in Pancreatic Cancer	Maria Teresa Rizzo	2003
12527803	Akt-Dependent Phoshorylation of Serine 1179 and Mitogen-Activated Protein Kinase Kinase/Extracellular Signal-Regulated Kinase 1/2 Cooperatively Mediate Activation of the endothelial Nitric-Oxcide Synthase by Hydrogen Peroxide	Hua Cai	2003
11090064	Secreted phospholipase A2 induces vascular endothelial cell migration	Maria Teresa Rizzo	2000

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