Human Brain Microvascular Endothelial Cells
Certificate #: ACBRI 376

Introduction

Primary Human Brain Microvascular Endothelial Cells (ACBRI 376) were initiated by elutriation of dispase dissociated normal human brain cortex tissue.

Cell Initiation

These cells were originated using CSC Complete Serum-Free Medium (SF-4Z0-500), are available at < 12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~ 1.5 X 106 cells. This vial will initiate a Passage 4 cell culture in a 75cm2 flask.

These cells are available in cryopreserved vials as well as in 25cm2 and 75cm2 proliferating cell culture flasks.

We have other individual donor lots available. Please contact us for details.
Enlarge Photo
Digital Phase-Contrast Image (40X) @ Passage 5

Companion Products

Each vial or flask of cells is shipped to Customer with Bac-Off® (antibiotic) and CultureBoost (animal derived growth factors) or CultureBoost-R (human recombinant growth factors) at no additional cost.

These cells are qualified for use with: CSC Complete Serum Free Medium (SF-4Z0-500) and CSC Complete Medium which includes 10% serum (4Z0-500); CSC Attachment Factor™ (4Z0-210); CSC Passage Reagent Group™ (4Z0-800) and CSC Cell Freezing Medium (4Z0-705).
Enlarge Photo
Digital Fluorescence Images (direct flourescence 400X, indirect immunofluoresence 6300X) @ Passage 5

Cell Growth Images

Cell growth images are available.

Standard Tests

TEST

RESULTS

HIV Serologic Test (donor level HIV AB EIA)

Negative

HIV PCR TEST (frozen cell pool by CLIA Licensed Clinical Lab)

Negative

Test of frozen cells for Mycoplasma spp. (ATCC method by CLIA Licensed Clinical Lab)

Negative

 

Miscellaneous Tests

TEST

RESULTS

Cytoplasmic VWF / Factor VIII

 > 95% positive by immunofluorescence

Cytoplasmic uptake of Di-I-Ac-LDL

 > 95% positive by immunofluorescence

 

Additional Information

These cells are involved in the blood-brain barrier, HIV-Aids pathology, inflammation, and interact in vivo with astrocyte cells (ACBRI 371) and pericyte cells (ACBRI 499)

Functional Test: IL-1ß Stimulated Leukocyte Adherence 

Primary Brain Microvascular Cells can provide a useful tool for adherence, transport and permeability studies of the blood-brain-barrier (BBB).  ACBRI 376 cells demonstrate particular markers of differentiation (interdigitated cell contact, desmosomes, (Z0-1 protein epitopes) similar to those observed in vivo.  The morphological and immunofluorescent markers detailed for ACBRI 376 are suggestive of a differentiated cell monolayer that can be used to study properties of the human BBB.  These cells are extensively used in studies of HIV transmission and AIDS-related BBB dynamics.
Enlarge Photo
Digital Immunofluorescence ZO-1 mAb (6300X) @ Passage 5

 

In a functional test, ACBRI 376 monolayers in CSC Serum-Free Maintenance Medium were tested for IL-1ß stimulated leukocyte adherence.  Human leukocyte adherence was specific and dose-dependent as demonstrated by pretreatment of the microvascular monolayer with soluble IL-1ß receptor.  Nonspecific leukocyte adherence caused by phorbol ester was the positive control.  Polymorphonuclear leukocytes (PMN) were freshly isolated from peripheral blood by elutriation.
Enlarge Photo
Response to recombinant human IL-1ß stimulation in a PMN adherence assay @ P5 in vitro.

Citations
 PMID  Title  Author  Year
21602932 Downregulation of FIP200 Induces Apoptosis of Glioblastoma Cells and Microvasular Endothelial Cells by Enhancing Pyk2 Activity Candece L. Gladson 2011
21562076 Decreased Cerebrovascular Brain-Derived Neurotrophic Factor-Mediated Neuroprotection in the Diabetc Brain Eng H. Lo 2011
 21151895 Phosphodiesterase-III Inhibitor Prevents Hemorrhagic Transformation Induced by Focal Cerebral Ischemia in Mice treated with tPA Mitsunori Ishiguro 2010
 21060661 Evaluation of Soluble Junctional Adhesion Molecule-A as a Biomarker of Human Brain Endothelial Barrier Breakdown Axel Haarmann 2010
  20952385 Brain Endothelial Cells Produce Amyloid beta From Amyloid Precursor Protein 770 And Preferentially Secrete The O-Glycosylated Form Shinobu Kitazume 2010
20647538 Single-Molecule Analysis Reveals Changes in the DNA Repliation Program for the POU5F1 Locus upon Human Embryonic Stem Cell Differentiation Sherri S. Schultz 2010
20519836 Resistance of human brain microvascular endothelial cells in culture to methylmercury: cell-density-dependent defense mechanisms Takashi Hirooka 2010
20371982 DNA microarray gene expression analysis of human vascular endothelial cells exposed to arsenite Yasuyuki Fujiwara 2010
 19948061 Characterization of TEM1/endosialin in human and murine brain tumors Eleanor Carson-Walter 2009
  19815818 Alzheimer Disease-Associate Peptide, Amyloid beta 40, Inhibits Vascular Regeneration With Induction of Endothelial Autophagy Shin-ichiro Hayashi 2009
 19397812 Soluble aggregates of the amyloid-beta peptide are trapped by serum albumin to enhance amyloid-beta activation of endothelial cells Melissa A Moss 2009
 19174516
VEGF-mediated disruption of endothelial CLN-5 promotes blood-brain barrier breakdown
Gareth R. John 2009
 18977327
miR-296 regulates growth factor receptor overexpression in angiogenic endothelial cells
Thomas Wurdinger 2008
 18941231
Cannabinoids Inhibit HIV-1 Gp120-Mediated Insults in Brain Microvascular Endothelial Cells
Tzong-Shi Lu 2008
 18726995
Thrombospondin-1-induced apoptosis of brain microvascular endothelial cells can be mediated by TNF-R1
Candece L. Gladson 2009
 18702666 Soluble aggregates of the amyloid-beta protein selectively stimulate permeability in human brain microvascular endothelial monolayers Melissa A. Moss 2008
 18599607
Hypoxia- and Vascular Endothelial Growth Factor-Induced Stromal Cell-Derived Factor-1alpha/CXCR4 Expression in Glioblastomas
David Zagzag 2008
 18354059
Mitochondrial Effects of Estrogen Are Mediated by Estrogen Receptor alpha in Brain Endothelial Cells
Ali Razmara 2008
 18535159 MRP (ABCC) Transporters-Mediated Efflux of Anti-HIV Drugs, Saquinavir and Zidovudine, from Human Endothelial Cells Debasis Mondal 2008
 18302767 Alterations in the Notch4 parhway in cerebral endothelial cells by the HIV aspartyl protease inhibitor, nelfinavir. T Diane Langord

 

2008
 17953673 Soluble aggregates of amyloid-beta protein activate endothelial monolayers for adhesion and subsequent transmigration of monocyte cells Melissa A. Moss 2007
 17399760 Polyomavirus JC Infects Human Brain Microvascular Endothelial Cells Independent og Serotonin Receptor 2A Moti L. Chapagain 2007
 17442832 AKAP12 Regulates Human Blood-Retinal Barrier Formation by Dounregulation of Hypoxia-Inducible Factor 1 alpha Yoon Kyung Choi 2007
 17085437 Integrin Regulation by Vascular Endothelial Growth Factor in Human Brain Vicrovascular Endothelial Cells Shalom Avraham 2006
 17075581  Hypoxia-inducible factor 1 and VEGF upregulate CXCR4 in glioblastoma: implications for angiogenesis and glioma cell invasions David Zagzag 2006
 17015745 IL-1beta Regulates Blood-Brain Barrier Permeability via Reactivation of the Hypoxia Angiogenesis Program Gareth R. John 2006
 16436595 CCL2/Monocyte Chemoattractant Protein-1 Mediates Enhanced Transmigration of Human Immunodeficienct Virus (HIV)-Infected Leukocytes across the Blood-Brain Barier: A Potential Mechanism of Hiv-CNS Invasion and NeuroAIDS Joan W Berman 2006 
 15767427 Human Immunodeficiency Virus Type 1 Nef Potently Induces Apoptosis in Primary Human Brain Microvascular Endothelial Cells via the Activation of Caspases Edward A. Acheampong 2005
 15800057 Arginine vasopressin stimulation of cerebral microvascular endothelial cell Na-K-Cl cotransporter activity is V1 receptor and (Ca) dependent Martha E.  O'Donnell 2005
 15528360 HIV-1 Tat-Mediated Effects on Focal Adhesion Assembly and Permeability in Brain Microvascular Endothelial Cells Shalom Avraham 2004
15684470  Selective Promotion of Plasminogen Acivator Inhibitor-1 Secretion by Activation of Proteinase-Activated Receptor-1 Cultured Human Brain Microvascular Pericytes: Comparison with Endothelial Cells  Toshiyuki Kaji  2004
 15163749 Contribution of Proteoglycans to Human Immunodeficiency Virus Type 1 Brain Invasion Michael D. Bobardt 2004
 12682234 Expression of the NF-kB Target Gene X-Ray-Inducible Immediate Early Response Factor-1 Shor Enhances TNF alpha-Induced Hepatocyte Apoptosis by Inhibiting Akt Activation Yosuke Osawa 2003 
 14551401 Interactions Between p38 Mitogen-Activated Protein Kinase and Caspase-3 in Cerebral Endothelial Cell Death After Hypoxia-Reoxygenation Sun-Ryung Lee 2003
 12844492 Vascular Endothelial Growth Factor Regulates Focal Adhesion Assembly in Human Brain Microvascular Endothelial Cells through Activation of the Focal Adhesion Dinase and Related Adhesion Focal Tyrosine Kinase Shalom Avraham 2003
 12594291 HIV-1 Tat-Mediated Apoptosis in Human Brain Microvascular Endothelial Cells Shalom Avraham 2003
 12757771 Endothelial cells release phenotypically and quantitatively distinct microparticles in activation and apoptosis Joaquin J. Jimenez 2003
 15618195 mRNA Expression and Amino Acid Transport Characteristics of Cultured Human Brain Microvascualr Endothelial Cells (hBME) Nouo Umeki 2002
 12446667 Vascular Endothelial Growth Factor Modulates the Transenothelial Migration of MDA-231 Breast Cancer Cells through Regulation of Brain Microvascular Endothelial Cell Permeability Shalom Avarham 2002
 11847215 Neutrophil-derived Glutamate Regulates Vascular Endothelial Barrier Function Charles D. Collard 2002
 12191968 Molecular Basis for High Renel Cell Sensitivity to the Cytotoxic Effects of Shigatoxin-1: Upregulation of Globotriaosylceramide Expression Donald E. Kohan 2002
 11784713 Vascular Endothelial Growth Factor Modulates Neutrophil Transendothelial Migration via Up[regulation of Interleukin-8 in Human Brain Microvascular Endothelial Cells Shalom Avraham 2002
 10985260 Induction of cyclooxygenase-2 expression during HIV-1 infected monocyte-derived macrophage and human brain microvascular endothelial cell interaction. Candida F. Pereira 2000


ACBRI is a nonprofit research institute providing research support to biomedical and cell biology institutions/agencies/organizations. CSC is the authorized agent for distribution of ACBRI research material. Funds collected by CSC for ACBRI products are transferred to ACBRI to cover acquisition and research support costs. 

IRB acquisition requirements prohibit involvement of ACBRI research material in any profit-making enterprise, and Cell Systems Corporation does not profit from handling or delivery of ACBRI products. There are specific restrictions on the employment of ACBRI cells. ACBRI cells are for research purposes only and may not be transferred out of the direct control of the Recipient Institution/agency/organization. ACBRI cells may not be genetically altered in any way without prior written permission by ACBRI. Use of ACBRI products (as documented by placement of any order for product by an authorized representative constitutes knowledge, understanding and binding acceptance of these restrictions on behalf of the recipient organization/institution. 

Please read our Terms and Conditions. 

 
   

Copyright © 1997-2010 Cell Systems Corporation. All Rights reserved