Attachment Factor, for Glass Cover Slide

Certificate #: 4Z0-210-S

Introduction

Attachment Factor™ (4Z0-210-S) is an extracellular matrix (ECM) product specifically designed for use with glass rather than plastic. This product promotes cell attachment to glass tissue culture surfaces and encourages correct polarity and cytoskeletal organization. 4Z0-500-S also contributes to correct in vitro growth factor and biological response modifier presentation.

The biologically active elements of Attachment Factor™ are derived by a chromatographic process from heat-inactivated charcoal-stripped fetal bovine serum, and delivered at 1X in a PBS based HEPES-buffered collagen vehicle. CSC exclusively uses and strongly recommends the use of serum products 100% traceable to cattle processed in the US at USDA inspected facilities.

Purpose

Use of Attachment Factor™ improves plating efficiency, shortens "lag time" after passage, enhances growth rates, and promotes the organization of polarity and cytoskeletal architecture, and can dramatically increase cell yield during cell culture expansion. Use with all cultures EXCEPT Melanocyte and Keratinocyte cells.

Use

Use of Attachment Factor™ in passaging cells:

1. Follow the PRG instructions for preparation of the cells for subculture.

2. Wet the tissue culture surface(s) with warmed Attachment Factor™ (at 37°C).

3. Remove the excess Attachment Factor™ and add CSC Complete Medium.

4. The surface is activated for use immediately: rinsing or drying are not required or recommended.

5. Inoculate the culture at once according to the Medium Kit instructions.

6. Incubate at 37°C, 5% CO₂, 100% humidity. Feed according to Kit instructions.

Use of Attachment Factor™ in thawing cells:

1. Refer to the PRG Instructions for subculture and Cell Freezing Instructions for thawing.

2. Prepare the new culture surface using Attachment Factor™.

(a) Warm Attachment Factor™ to 37°C in the water bath.

(b) Wet the culture surface to be inoculated with Attachment Factor™.

3. The culture may be inoculated at once.

Citations

PMID	Title	Author	Year
20110406	Inhibition of Protein Kinase C o Attenuates Blood-Retinal Barrier in Diabetic Retinopathy	Jeong-Hun Kim	2010
19875648	Protective Effect of Clusterin on Blood-Retinal Barrier Breakdown in Diabetic Retinopathy	Jeong-Hun Kim	2010
19756180	Decursin inhibits retinal neovascularization via suppression of VEGFR-2 activation	Jeong Hun Kim	2009
19397812	Soluble aggregates of the amyloid-beta peptide are trapped by serum albumin to enhance amyloid-beta activation of endothelial cells	Melissa A Moss	2009
18702666	Soluble aggregates of the amyloid-beta protein selectively stimulate permeability in human brain microvascular endothelial monolayers	Melissa A. Moss	2008
17959724	Shear stress and 17 beta-estradiol modulate cerebral microvascular endothelial Na-K-Cl cotransporter and Na/H exchanger protein levels	Abdul I. Barakat	2008
17953673	Soluble aggregates of amyloid-beta protein activate endothelial monolayers for adhesion and subsequent transmigration of monocyte cells	Melissa A. Moss	2007
17442832	AKAP12 Regulates Human Blood-Retinal Barrier Formation by Dounregulation of Hypoxia-Inducible Factor 1 alpha	Yoon Kyung Choi	2007
17399760	Polyomavirus JC Infects Human Brain Microvascular Endothelial Cells Independent og Serotonin Receptor 2A	Moti L. Chapagain	2007
17015745	IL-1beta Regulates Blood-Brain Barrier Permeability via Reactivation of the Hypoxia Angiogenesis Program	Gareth R. John	2006
16352855	Role of fibroblast growth factor-binding protein in the pathogenesis of HIV-associated helolytic uremic syndrome	Patricio E. Ray	2006
14610228	Dexamethasone-Mediated Up-Regulation of the Mannose Receptor Improves the Delivery of Recombinant Glucocerebrosidase to Gaucher Macrophages	Yunsiang Zhu	2003
14551401	Interactions Between p38 Mitogen-Activated Protein Kinase and Caspase-3 in Cerebral Endothelial Cell Death After Hypoxia-Reoxygenation	Sun-Ryung Lee	2003
12844492	Vascular Endothelial Growth Factor Regulates Focal Adhesion Assembly in Human Brain Microvascular Endothelial Cells through Activation of the Focal Adhesion Dinase and Related Adhesion Focal Tyrosine Kinase	Shalom Avraham	2003
12757771	Endothelial cells release phenotypically and quantitatively distinct microparticles in activation and apoptosis	Joaquin J. Jimenez	2003
12670949	Beta3-Adrenergic Receptors Regulate Retinal Endothelial Cell Migration and Proliferation	Jena J. Steinle	2003
12533667	Potential Involvement of the Cyclooxygenase-2 Pathwya in the Regulation of Tumor-associated Angiogenesis and Growth in Pancreatic Cancer	Maria Teresa Rizzo	2003
12446667	Vascular Endothelial Growth Factor Modulates the Transenothelial Migration of MDA-231 Breast Cancer Cells through Regulation of Brain Microvascular Endothelial Cell Permeability	Shalom Avarham	2002
12213726	Abnormal Uterine Bleeding during Progestin-Only Contraception May Result from Free Radical-Induced Alterations in Angiopoietin Expression	Graciela Krikun	2002
11784713	Vascular Endothelial Growth Factor Modulates Neutrophil Transendothelial Migration via Up[regulation of Interleukin-8 in Human Brain Microvascular Endothelial Cells	Shalom Avraham	2002
11090064	Secreted phospholipase A2 induces vascular endothelial cell migration	Maria Teresa Rizzo	2000
10684811	Human Endomertrial Cells: Isolation, Characterization and Inflammatory-Mediated Expression of Tissue Factor and Type 1 Plaminogen Activator Inhibitor	Frederick Schatz	2000
9122172	Aputative angiogenin receptor in angiogenin-responsive human endothelial cells	Guo-fu Hu	1997

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