Bovine Pulmonary Artery Endothelial Cells

Certificate #: CSC 2BA3

Introduction

Primary Bovine Pulmonary Artery Endothelial Cells (CSC 2BA3) are pooled primary isolates from seven individual donor veal calf pulmonary arteries.

These cells were originated using CSC Complete Serum-Free Medium (SF-4Z0-500), are available at < 12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~ 1.5 X 106 cells.  This vial will initiate a Passage 4 cell culture in a 75cm2 flask.

These cells are available in both cryopreserved vials and in 75cm2 proliferating culture flasks.

Each vial or flask of cells is shipped to Customer with Bac-Off® (antibiotic) and CultureBoost (animal derived growth factors) or Cultureboost-R (human recombinant growth factors) at no additional cost.  

These cells are qualified for use with all recombinant and non-recombinant versions of CSC Serum-Free and Serum-Containing (10% by volume) Complete Medium Kits; CSC Attachment Factor™ (4Z0-210); CSC Passage Reagent Group™ (4Z0-800) and CSC Cell Freezing Medium (4Z0-705).

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Bovine Pulmpnary Artery Endothelial Cells Catalog CSC 2BA3 Passage 9 40X Phase Contrast Dgital Image

Primary Bovine Pulmonary Artery Endothelial Cell System Proliferation Assay
12-26 CPD in vitro /  Passages 4-9
CSC 2BA3 Bovine Pulmonary Artery Endothelial Cells
4Z0-500 Complete Serum Containing Medium Kit
4Z0-800 Passage Reagent Group™ (PRG)
CSC specifies bovine serum and serum-derived components 100% traceable to cattle processed in the US at USDA-inspected facilities.

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Standard Tests

Test Results
HIV PCR TEST (frozen cell pool by CLIA Licensed Clinical Lab)  Negative
Test of frozen cells for Mycoplasma spp. (ATCC method by CLIA Licensed Clinical  Lab)  Negative

 
   USDA Form 16-4 (Declaration Regarding health of the Donor Animal) May be required for some international orders. If Recipient's government requires a 16-4 Declaration for animal products, Recipient will be responsible for obtaining information from his/her Customs Officials for use by the USDA in determining whether a Form 16-4 can be issued.

Citations
PMID Title Author Year
 15941713     Vascular Endothelial Growth Factor Receptor-2 Counter-Regulation by the Transcription Factors, TFII-I and TFII-IRD1  Tanisha A Jackson  2005
 11091480  High-efficiency transient transfection of endothelial cells for functional analysis  Denis English  2000

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