Bovine Brain Microvascular Endothelial Cells

Certificate #: CSC 2BB3

Introduction

These cells were originated using CSC Complete Serum-Free Medium (SF-4Z0-500), are available at < 12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~ 1.5 X 106 cells.  This vial will initiate a Passage 4 cell culture in a 75cm2 flask.

These cells are available in both cryopreserved vials and in 75cm2 proliferating culture flasks.

Each vial or flask of cells is shipped to Customer with Bac-Off® (antibiotic) and CultureBoost (animal derived growth factors) or Cultureboost-R (human recombinant growth factors) at no additional cost.

These cells are qualified for use with all recombinant and non-recombinant versions of CSC Serum-Free and Serum-Containing (10% by volume) Complete Medium Kits; CSC Attachment Factor™ (4Z0-210); CSC Passage Reagent Group™ (4Z0-800) and CSC Cell Freezing Medium (4Z0-705).

 

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Bovine Brain Microvascular Endothelial Cells Catalog CSC 2BB3 Passage 5 40X Phase Contrast Dgital Image

Primary Bovine Brain Microvascular Endothelial Cell System Proliferation Assay
9-28 CPD in vitro  /  Passages 3-8
CSC 2BB3 Primary Bovine Brain Mv Endothelial Cells
4Z0-500 Complete Serum Containing Medium Kit
4Z0-800 Passage Reagent Group™ (PRG)
CSC specifies bovine serum and serum-derived components 100% traceable to cattle processed in the US at USDA-inspected facilities.

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Standard Tests

Test Results
HIV PCR TEST (frozen cell pool by CLIA Licensed Clinical Lab)  Negative
Test of frozen cells for Mycoplasma spp. (ATCC method by CLIA Licensed Clinical  Lab)  Negative

 
   USDA Form 16-4 (Declaration Regarding health of the Donor Animal) May be required for some international orders. If Recipient's government requires a 16-4 Declaration for animal products, Recipient will be responsible for obtaining information from his/her Customs Officials for use by the USDA in determining whether a Form 16-4 can be issued.

Citations
PMID Title Author Year
19174516
VEGF-mediated disruption of endothelial CLN-5 promotes blood-brain barrier breakdown
Gareth R. John 2009
 16079189  Moderate-to-severe ischemic conditions increase activity and phosphorylation of the cerebral microvascular endothelial cell Na+-K+-C1 contransporter  Martha E. O'Donnell  2005
 15800057  Arginine vasopressin stimulation of cerebral microvascular endothelial cell Na-K-Cl cotransporter activity is V1 receptor and (Ca) dependent  Martha E.  O'Donnell  2005
 11157197  Caspase Inhibitors Attenuate Oxyhemoglobin-Induced Apoptosis in Endothelial Cells  Toshinari Meguro  2001 
 11325019  Na+/H+ Exchange Inhibitor SM-20220 Improves Endothelial Dysfunction Induced by Ischemia-Reperfusion  Naohito Ohashi  2000
 16982627  Redox Regulation of 4-Hydroxy-2-nonenal-mediated Endothelial Barrieer Dysfunction by Focal Adhesion, Adherens, and Tight Junctions Proteins  Peter V. Ustyuk  2006
 11053247  Sphingosine1-phospahate released from platelets during clotting accounts for the porent endothelial cell cheotactic activity of blood serum and provides a novel link between hemostasis and angiogenesis  Denis English  2000

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