Bovine Pulmonary Microvascular Endothelial Cells

Certificate #: CSC 2BM3

Introduction

Primary Bovine Pulmonary Microvascular Endothelial Cells (CSC 2BM3) are pooled primary isolates from ten individual donor veal calf brain peripheral lungs.

These cells were originated using CSC Complete Serum-Free Medium (SF-4Z0-500), are available at < 12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~ 1.5 X 106 cells.  This vial will initiate a Passage 4 cell culture in a 75cm2 flask.

These cells are available in both cryopreserved vials and in 75cm2 proliferating culture flasks.

Each vial or flask of cells is shipped to Customer with Bac-Off® (antibiotic) and CultureBoost (animal derived growth factors) or Cultureboost-R (human recombinant growth factors) at no additional cost.

These cells are qualified for use with all recombinant and non-recombinant versions of CSC Serum-Free and Serum-Containing (10% by volume) Complete Medium Kits; CSC Attachment Factor™ (4Z0-210); CSC Passage Reagent Group™ (4Z0-800) and CSC Cell Freezing Medium (4Z0-705).

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Bovine Pulmonary Microvascular Endothelial Cells Catalog CSC 2BM3 Passage 8 100X Phase Contrast Dgital Image

Primary Bovine Pulmonary Microvascular Endothelial Cell System Proliferation Assay
9-28 CPD in vitro  /  Passages 3-10
CSC 2BM3 Primary Bovine Pulmonary Mv Endothelial Cells
4Z0-500 Complete Serum Containing Medium Kit
4Z0-800 Passage Reagent Group™ (PRG)
CSC specifies bovine serum and serum-derived components 100% traceable to cattle processed in the US at USDA-inspected facilities.

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Standard Tests

Test Results
HIV PCR TEST (frozen cell pool by CLIA Licensed Clinical Lab)  Negative
Test of frozen cells for Mycoplasma spp. (ATCC method by CLIA Licensed Clinical  Lab)  Negative

 
   USDA Form 16-4 (Declaration Regarding health of the Donor Animal) May be required for some international orders. If Recipient's government requires a 16-4 Declaration for animal products, Recipient will be responsible for obtaining information from his/her Customs Officials for use by the USDA in determining whether a Form 16-4 can be issued.

PMID Title Author Year
18987163 Effects of Extracellular ATP on Bovine Lung Endothelial and Epithelial Monolayer Morphologies, Apoptoese and Permeabilities David McClenahan 2009
16982627 Redos Regulation of 4-Hydroxy-2-nonenal-mediated Endothelial Barrier Dysfunction by Focal Adhesion, Adherens,and Tight Junction Proteins Peter V. Usatyuk 2006

 

 

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