Porcine Liver Microvascular Endothelial Cells

Certificate #: CSC 2PL3

Introduction

Primary Porcine Liver Microvascular Endothelial Cells (CSC 2PL3) are pooled primary isolates from dispase dispersed peripheral neonatal porcine liver tissue from four individual donor animals, and were initiated by elutriation.

These cells were originated using CSC Complete Medium (4Z0-500), are available at < 12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~ 1.5 X 106 cells.  This vial will initiate a Passage 4 cell culture in a 75cm2 flask.

These cells are available in both cryopreserved vials and in 75cm2 proliferating culture flasks.

Each vial or flask of cells is shipped to Customer with Bac-Off® (antibiotic) and CultureBoost (animal derived growth factors) or Cultureboost-R (human recombinant growth factors) at no additional cost.

These cells are qualified for use with all recombinant and non-recombinant versions of CSC Serum-Free and Serum-Containing (10% by volume) Complete Medium Kits; CSC Attachment Factor™ (4Z0-210); CSC Passage Reagent Group™ (4Z0-800) and CSC Cell Freezing Medium (4Z0-705).

Porcine Liver Microvascular Endothelial Cells Catalog CSC 2PL3

Porcine Liver Microvascular Endothelial Cell System Proliferation Assay
12-30 CPD in vitro  /  Passages 4-10
Catalog CSC 2PL3
Porcine Liver Microvascular Cells
Catalog 4Z0-500 Complete Serum Containing Medium Kit
Catalog 4Z0-800 Passage Reagent Group™
CSC specifies bovine serum and serum-derived components 100% traceable to cattle processed in the US at USDA-inspected facilities.

Enlarge Photo

Standard Tests

Test Results
Test of frozen cells for Mycoplasma spp. (ATCC method by CLIA Licensed Clinical Lab) Negative


   USDA Form 16-4 (Declaration Regarding health of the Donor Animal) may be required for some international orders. If Recipient's government requires a 16-4 Declaration for animal products, Recipient will be responsible for obtaining information from his/her Customs Officials for use by the USDA in determining whether a Form 16-4 can be issued.

Miscellaneous Tests

Test Result
Cytoplasmic VWF / Factor VIII Cytoplasmic VWF / Factor VIII >90% positive by immunofluorescenceVIII
Cytoplasmic VWF / Factor VIII

Cytoplasmic VWF / Factor VIII >90% positive by immunofluorescenceVIII

Cytoplasmic uptake of Di-I-Ac-LDL Cytoplasmic uptake of Di-I-Ac-LDLLDL >95% positive by immunofluorescence
Test for Cytokeratin Intermediate Filaments by indirect immunofluorescence Cytoplasmic uptake of Di-I-Ac-LDLLDL <95% positive by immunofluorescence

 

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