Human Venous Endothelial Cells

Certificate #: CSC 2V0

Introduction

Primary Human Venous Endothelial Cells (CSC 2V0) are pooled primary isolates from 250 individual human donor umbilical veins.

These cells were originated using CSC Complete Serum-Free Medium (SF-4Z0-500), are available at < 12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~ 1.5 X 106 cells.  This vial will initiate a Passage 4 cell culture in a 75cm2 flask.

These cells are available in both cryopreserved vials as well as in 25cm2 and 75cm2 proliferating culture flasks.

Each vial or flask of cells is shipped to Customer with Bac-Off® (antibiotic) and CultureBoost (animal derived growth factors) or Cultureboost-R (human recombinant growth factors) at no additional cost.

These cells are qualified for use with all recombinant and non-recombinant versions of CSC Serum-Free and Serum-Containing (10% by volume) Complete Medium Kits; CSC Attachment Factor™ (4Z0-210); CSC Passage Reagent Group™ (4Z0-800) and CSC Cell Freezing Medium (4Z0-705).

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Human Venous Endothelial Cells Catalog CSC 2V0 Passage 5 40X Phase Contrast Digital Image

Primary Human Venous Endothelial Cell Proliferation Assay
3-23 CPD in vitro /  Passages 1-8
Catalog CSC 2V0 Human Venous Endothelial Cells
Catalog 4Z0-500 Complete Serum Containing Medium Kit
Catalog 4Z0-210 Attachment Factor™
Catalog 4Z0-800 Passage Reagent Group™
CSC specifies bovine serum and serum-derived components 100% traceable to cattle processed in the US at USDA-inspected facilities.
  

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Cell Growth Images

Cell growth images are available.

Standard Tests

Test Results
 HIV Serologic Test (donor level HIV AB EIA)  Negative
 HIV PCR TEST (frozen cell pool by CLIA Licensed Clinical Lab)  Negative
 Test of frozen cells for Mycoplasma spp. (ATCC method by CLIA Licensed Clinical  Lab)  Negative

 

Miscellaneous Tests 

 

Test

Result

Cytoplasmic VWF / Factor VIII

Cytoplasmic VWF / Factor VIII < 95% positive by immunofluorescence VIII

Cytoplasmic uptake of Di-I-Ac-LDL

Cytoplasmic uptake of Di-I-Ac-LDL < 95% positive by immunofluorescence

 

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