Human Microvascular Endothelial Cells

Certificate #: CSC 2M1

Introduction

Primary Human Microvascular Endothelial Cells (CSC 2M1) are pooled primary isolates from 250 individual neonatal human dermal donor isolates.

These cells were originated using CSC Complete Medium (4Z0-500), are available at < 12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~ 1.5 X 10⁶ cells. This vial will initiate a Passage 4 cell culture in a 75cm² flask.

These cells are available in both cryopreserved vials as well as in 25cm² and 75cm² proliferating culture flasks.

Each vial or flask of cells is shipped to Customer with Bac-Off^® (antibiotic) and CultureBoost (animal derived growth factors) or Cultureboost-R (human recombinant growth factors) at no additional cost.

These cells are qualified for use with all recombinant and non-recombinant versions of CSC Serum-Free and Serum-Containing (10% by volume) Complete Medium Kits; CSC Attachment Factor™ (4Z0-210); CSC Passage Reagent Group™ (4Z0-800) and CSC Cell Freezing Medium (4Z0-705).

Primary Human Mv Endothelial Cells Catalog CSC 2M1 Passage 4 40X Phase Contrast Digital Image

Primary Human Microvascular Endothelial Cell System Proliferation Assay
3-23 CPD in vitro / Passages 1-8
Human Microvascular Endothelial Cells
Catalog 4Z0-500 Complete Serum Containing Medium Kit
Catalog 4Z0-210 Attachment Factor™
Catalog 4Z0-800 Passage Reagent Group™
CSC specifies bovine serum and serum-derived components 100% traceable to cattle processed in the US at USDA inspected facilities.

Standard Tests

Test	Results
HIV Serologic Test (donor level HIV AB EIA)	Negative
HIV PCR TEST (frozen cell pool by CLIA Licensed Clinical Lab)	Negative
Test of frozen cells for Mycoplasma spp. (ATCC method by CLIA Licensed Clinical Lab)	Negative

Miscellaneous Tests

Test	Result
Cytoplasmic VWF / Factor VIII	Cytoplasmic VWF / Factor VIII > 95% positive by immunofluorescence^VIII
Cytoplasmic uptake of Di-I-Ac-LDL	Cytoplasmic uptake of Di-I-Ac-LDL > 95% positive by immunofluorescence
Inducible expression of CD36	Inducible expression of CD36 > 95% positive by immunofluorescence
Formation of Capillary-like Structures on EHS Sarcoma ECM	Formation of Capillary-like Structures on EHS Sarcoma ECM Positive

Citations

PMID	Title	Author	Year
17491018	Anthrax Edema Toxin Inhibits Endothelial Cell Chemotaxis via Epac and Rap1	Jia Hong	2007
15161644	Endometrial Angiopoietin Expression and Modulation by Thrombin and Steroid Hormones	Graciela Krikun	2004
12213726	Abnormal Uterine Bleeding during Progestin-Only Contraception May Result from Free Radical-Induced Alterations in Angiopoietin Expression	Graciela Krikun	2002
11292666	Antiangiogenesis signals by endostatin	Masayoshi Shichiri	2001
9122172	Aputative angiogenin receptor in angiogenin-responsive human endothelial cells	Guo-fu Hu	1997

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