Human Smooth Muscle Cells

Certificate #: CSC 2SM3

Introduction

Primary Human Vascular Smooth Muscle Cells (CSC 2SM3) are pooled primary isolates from 250 individual human neonatal donor umbilical arteries.

These cells were originated using CSC Complete Medium (4Z0-500), are available at < 12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~ 1.5 X 106 cells.  This vial will initiate a Passage 4 cell culture in a 75cm2 flask.

These cells are available in both cryopreserved vials as well as in 25cm2 and 75cm2 proliferating culture flasks.

Each vial or flask of cells is shipped to Customer with Bac-Off® (antibiotic) and CultureBoost (animal derived growth factors) or Cultureboost-R (human recombinant growth factors) at no additional cost.

These cells are qualified for use with all recombinant and non-recombinant versions of CSC Serum-Free and Serum-Containing (10% by volume) Complete Medium Kits; CSC Attachment Factor™ (4Z0-210); CSC Passage Reagent Group™ (4Z0-800) and CSC Cell Freezing Medium (4Z0-705).

Primary Human Vascular Smooth Muscle Cells Catalog CSC 2SM3 Passage 6 40X Phase Contrast Digital Image

Primary Human Vascular Smooth Muscle Cell System Proliferation Assay
12-26 CPD in vitro /  Passages 4-9
Catalog CSC 2SM3
Human Vascular Smooth Muscle Cells
4Z0-500 Complete Serum Containing Medium Kit
Attachment Factor™and Passage Reagent Group™
CSC specifies bovine serum and serum-derived components 100% traceable to cattle processed in the US at USDA-inspected facilities.

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Standard Tests

Test Results
 HIV Serologic Test (donor level HIV AB EIA)  Negative
 HIV PCR TEST (frozen cell pool by CLIA Licensed Clinical Lab)  Negative
 Test of frozen cells for Mycoplasma spp. (ATCC method by CLIA Licensed Clinical  Lab)  Negative

 

Miscellaneous Tests

Test Result
Cytoplasmic Desmin Intermediate Filaments Cytoplasmic Desmin Intermediate Filaments < 95% positive by immunofluorescence
Cytoplasmic SMC-Specific Actin Cytoplasmic SMC-Specific Actin < 95% positive by immunofluorescence

 

 

Citations

PMID Title Author Year
10684811 Human Endomertrial Cells: Isolation, Characterization and Inflammatory-Mediated Expression of Tissue Factor and Type 1 Plaminogen Activator Inhibitor Frederick Schatz 2000

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