Human Fibroblast Cells

Certificate #: CSC 2F0

Introduction

Primary Human Fibroblast Cells (CSC 2F0) are pooled primary isolates from 250 individual neonatal donor foreskins.

These cells were originated using CSC Complete Serum-Free Medium (SF-4Z0-500), are available at < 12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~ 1.5 X 10⁶ cells. This vial will initiate a Passage 4 cell culture in a 75cm² flask.

These cells are available in both cryopreserved vials as well as in 25cm² and 75cm² proliferating culture flasks.

Each vial or flask of cells is shipped to Customer with Bac-Off^® (antibiotic) and CultureBoost (animal derived growth factors) or Cultureboost-R (human recombinant growth factors) at no additional cost.

These cells are qualified for use with all recombinant and non-recombinant versions of CSC Serum-Free and Serum-Containing (10% by volume) Complete Medium Kits; CSC Attachment Factor™ (4Z0-210); CSC Passage Reagent Group™ (4Z0-800) and CSC Cell Freezing Medium (4Z0-705).

Primary Human Fibroblast Cells Catalog CSC 2F0 Passage 5 40X Phase Contrast Digital Image

Primary Human Fetal Lung Fibroblast CellSystem Proliferation Assay
15-34 CPD in vitro / Passages 5-11
Catalog CSC 2FF4 Human Fetal Lung Fibroblast Cells
Catalog 4Z0-500 CSC Complete Serum Containing Medium Kit
Catalog 4Z0-210 Attachment Factor™
Catalog 4Z0-800 Passage Reagent Group™
CSC specifies bovine serum and serum-derived components 100% traceable to cattle processed in the US at USDA-inspected facilities.

Standard Tests

Test	Results
HIV Serologic Test (donor level HIV AB EIA)	Negative
HIV PCR TEST (frozen cell pool by CLIA Licensed Clinical Lab)	Negative
Test of frozen cells for Mycoplasma spp. (ATCC method by CLIA Licensed Clinical Lab)	Negative

Miscellaneous Tests

Test	Result
Vimentin Intermediate Filaments	Vimentin Intermediate Filaments < 95% positive by immunofluorescence

Citations
PMID	Title	Author	Year
12787887	Novel anti-inflammatory actions of nobiletin, a citrus polymethoxy flavonoid on human synovial fibroblasts and mouse macrophages	Akira Ito	2002
12527793	A rapid biosensor chip assay for measuring of telomerase activity using surface plasmon resonance	Chihaya Maesawa	2003
12016152	Zerumbone, a Southeast Asian ginger sesquiterpene, markedly suppresses free radical generation, proinflammartory protein production, and cancer cell proliferation accompanied by apaptosis:the alpha, beta unsaturated carbonyl group is a prerequisite	Akira Murakami	2002

Please read our Terms and Conditions.