Human Epidermal Keratinocyte Cells

Certificate #: CSC 2K0

Introduction

Primary Human Keratinocyte Cells (CSC 2K0) are pooled primary isolates from 250 individual neonatal donor foreskins.

These cells were originated using CSC 2.0 Complete Serum-Free Medium (SF-4K0-500), are available at < 12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~ 1.5 X 106 cells.  This vial will initiate a Passage 4 cell culture in a 75cm2 flask.

These cells are available in both cryopreserved vials as well as in 25cm2 and 75cm2 proliferating culture flasks.

Each vial or flask of cells is shipped to Customer with Bac-Off® (antibiotic) and CultureBoost (animal derived growth factors) or Cultureboost-R (human recombinant growth factors) at no additional cost.

These cells are qualified for use with: CSC 2.0 Complete Serum Free Medium (SF-4K0-500); CSC Passage Reagent Group™ (4Z0-800) and CSC Cell Freezing Medium (4Z0-705).

Primary Human Keratinocyte Cells Catalog CSC 2K0

Primary Human Keratinocyte Serum-Free Cell System Proliferation Assay
3-26 CPD in vitro /  Passages 1-8
Catalog CSC 2K0 Primary Human Keratinocyte Cells
Catalog SF-4K0-500 Complete Serum-Free Maintenance Medium Kit
Catalog 4Z0-800 Passage Reagent Group™  
CSC specifies bovine serum and serum-derived components 100% traceable to cattle processed in the US at USDA-inspected facilities.

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Standard Tests

Test Results
 HIV Serologic Test (donor level HIV AB EIA)  Negative
 HIV PCR TEST (frozen cell pool by CLIA Licensed Clinical Lab)  Negative
 Test of frozen cells for Mycoplasma spp. (ATCC method by CLIA Licensed Clinical  Lab)  Negative

 

Miscellaneous Tests

Test Result
Test for Cytokeratin Intermediate Filaments by indirect immunofluorescence Test for Cytokeratin Intermediate Filaments by indirect immunofluorescence mAb to Cytokeratin, R-phycoerythrin

 

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