Human Small Intestine Epithelial Cells

Certificate #: ACBRI 519

Introduction

Primary Human Intestinal Epithelial Cells (ACBRI 519) were initiated by elutriation of dispase dissociated normal human small intestine tissue.

Cell Initiation

These cells were originated using CSC Complete Serum-Free Medium (SF-4Z0-500), are available at < 12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~ 1.5 X 10⁶ cells. This vial will initiate a Passage 4 cell culture in a 75cm² flask.

These cells are available in cryopreserved vials as well as in 25cm² and 75cm² proliferating cell culture flasks.

Monolayer Culture Phase-Contrast Digital Image (40X) Passage 5

Companion Products

Each vial or flask of cells is shipped to Customer with Bac-Off^® (antibiotic) and CultureBoost (animal derived growth factors) or CultureBoost-R (human recombinant growth factors) at no additional cost.

These cells are qualified for use with: CSC Complete Serum Free Medium (SF-4Z0-500) and CSC Complete Medium which includes 10% serum (4Z0-500); CSC Attachment Factor™ (4Z0-210); CSC Passage Reagent Group™ (4Z0-800) and CSC Cell Freezing Medium (4Z0-705).

Cell Growth Images

Cell growth images are available.

Standard Tests

TEST	RESULTS
HIV Serologic Test (donor level HIV AB EIA)	Negative
HIV PCR TEST (frozen cell pool by CLIA Licensed Clinical Lab)	Negative
Test of frozen cells for Mycoplasma spp. (ATCC method by CLIA Licensed Clinical Lab)	Negative

Miscellaneous Tests

TEST	RESULTS
Cytoplasmic Vimentin Intermediate Filaments	> 02% positive by indirect immunofluorescence
Cytoplasmic Cytokeratin Intermediate Filament	> 95% positive by indirect immunofluorescence

Additional Information

Functional Test: Barrier Formation

Primary Human Intestinal Epithelial Cells can provide a useful tool for absorption and permeability studies of the small intestine. ACBRI 519 cells demonstrate markers of epithelial differentiation (microvilli, desmosomes, cellular interdigitation) similar to those observed in vivo. The morphological and immunofluorescent markers demonstrated by ACBRI 519 are suggestive of a differentiated monolayer barrier that may be used to predict the absorption and transport of drugs and compounds across the mucosal barrier of the human small intestine. Plated in CSC Serum-Free Maintenance Medium (SF-4ZM-500), ACBRI 519 monolayers were tested for active barrier formation as measured by 90-minute C-Albumin flux (apical to basolateral) across the filter insert. Generation of peroxide by addition of 0.1 U/mL glucose oxidase to apical aspect of cells 30 minutes prior to albumin loading served as the positive control.

PMID	Title	Author	Year
21109446	Enzymatic synthesis of 125/131I labled 8-hydroxyquinoline glucuronide and in vitro/in vivo evaluation of biological infulence	Reyhan Yesilagac	2011
20578839	Enzymatic Synthesis of Uracil Glucuronide, Labeling with 125/131I, and In Vitro Evaluation on Adenocarcinoma Cells	Ilker Emin Medine	2010

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IRB acquisition requirements prohibit involvement of ACBRI research material in any profit-making enterprise, and Cell Systems Corporation does not profit from handling or delivery of ACBRI products. There are specific restrictions on the employment of ACBRI cells. ACBRI cells are for research purposes only and may not be transferred out of the direct control of the Recipient Institution/agency/organization. ACBRI cells may not be genetically altered in any way without prior written permission by ACBRI. Use of ACBRI products (as documented by placement of any order for product by an authorized representative constitutes knowledge, understanding and binding acceptance of these restrictions on behalf of the recipient organization/institution.

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