Human Astrocyte Cells

Certificate #: ACBRI 371

Introduction

Primary Human Brain Astrocyte Cells (ACBRI 371) were initiated by elutriation of dispase dissociated normal human brain cortex tissue. Astrocytes represent the most numerous microglial cell population in the cortical brain: the name "Astrocyte" derives from the stellate morphology of the cells in vivo. Astrocyte cells may be involved in extracellular ionic regulation, response to injury, and interact with pericyte cells (ACBRI 499) and with donor-paired microvascular endothelial cells (ACBRI 401). ACBRI 371 Primary Human Brain Astrocyte Cells have their origin in the same donor tissue as ACBRI 401, Primary Human Brain Microvascular Endothelial Cells.

Cell Initiation

These cells were originated using CSC Complete Serum-Free Medium (SF-4Z0-500), are available at < 12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~ 1.5 X 10⁶ cells. This vial will initiate a Passage 4 cell culture in a 75cm² flask.

These cells are available in cryopreserved vials as well as in 25cm² and 75cm² proliferating cell culture flasks.

Companion Products

Each vial or flask of cells is shipped to Customer with Bac-Off^® (antibiotic) and CultureBoost (animal derived growth factors) or CultureBoost-R (human recombinant growth factors) at no additional cost.

These cells are qualified for use with: CSC Complete Serum Free Medium (SF-4Z0-500) and CSC Complete Medium which includes 10% serum (4Z0-500); CSC Attachment Factor™ (4Z0-210); CSC Passage Reagent Group™ (4Z0-800) and CSC Cell Freezing Medium (4Z0-705).

Standard Tests

TEST	RESULTS
HIV Serologic Test (donor level HIV AB EIA)	Negative
HIV PCR TEST (frozen cell pool by CLIA Licensed Clinical Lab)	Negative
Test of frozen cells for Mycoplasma spp. (ATCC method by CLIA Licensed Clinical Lab)	Negative

Miscellaneous Tests

TEST	RESULTS
Cytoplasmic Vimentin Intermediate Filaments	> 02% positive by immunofluorescence
Cytoplasmic GFAP Intermediate Filaments	> 95% positive by immunofluorescence

Citations
PMID	Title	Author	Year
21602932	Downregulation of FIP200 Induces Apoptosis of Glioblastoma Cells and Microvascular Endothelial Cells by Enhancing Pyk2 Activity	Candece L. Gladson	2011
21151895	Phosphodiesterase-III Inhibitor Prevents Hemorrhagic Transformation Induced by Focal Cerebral Ischemia in Mice Treated with tPA	Mitsunori Ishiguro	2010
17442832	AKAP12 Regulates Human Blood-Retinal Barrier Formation by Dounregulation of Hypoxia-Inducible Factor 1 alpha	Yoon Kyung Choi	2007
16891468	Microsomal prostaglandin E synthase-1 regulates human glioma cell growth via prostaglandin E2-dependent activation of type II protein kinase A	Maria Teresa Rizzo	2006

ACBRI is a nonprofit research institute providing research support to biomedical and cell biology institutions/agencies/organizations. CSC is the authorized agent for distribution of ACBRI research material. Funds collected by CSC for ACBRI products are transferred to ACBRI to cover acquisition and research support costs.

IRB acquisition requirements prohibit involvement of ACBRI research material in any profit-making enterprise, and Cell Systems Corporation does not profit from handling or delivery of ACBRI products. There are specific restrictions on the employment of ACBRI cells. ACBRI cells are for research purposes only and may not be transferred out of the direct control of the Recipient Institution/agency/organization. ACBRI cells may not be genetically altered in any way without prior written permission by ACBRI. Use of ACBRI products (as documented by placement of any order for product by an authorized representative constitutes knowledge, understanding and binding acceptance of these restrictions on behalf of the recipient organization/institution.

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