Human Adult Dermal Microvascular Endothelial Cells

Certificate #: ACBRI 539

Introduction

Primary Adult Human Dermal Microvascular Endothelial Cells (ACBRI 539) were initiated from normal human ventral abdominal skin tissue.

Cell Initiation

These cells were originated using CSC Complete Serum-Free Medium (SF-4Z0-500), are available at < 12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~ 1.5 X 10⁶ cells. This vial will initiate a Passage 4 cell culture in a 75cm² flask.

These cells are available in cryopreserved vials as well as in 25cm² and 75cm² proliferating cell culture flasks.

Companion Products

Each vial or flask of cells is shipped to Customer with Bac-Off^® (antibiotic) and CultureBoost (animal derived growth factors) or CultureBoost-R (human recombinant growth factors) at no additional cost.

These cells are qualified for use with: CSC Complete Serum Free Medium (SF-4Z0-500) and CSC Complete Medium which includes 10% serum (4Z0-500); CSC Attachment Factor™ (4Z0-210); CSC Passage Reagent Group™ (4Z0-800) and CSC Cell Freezing Medium (4Z0-705).

Standard Tests

TEST	RESULTS
HIV Serologic Test (donor level HIV AB EIA)	Negative
HIV PCR TEST (frozen cell pool by CLIA Licensed Clinical Lab)	Negative
Test of frozen cells for Mycoplasma spp. (ATCC method by CLIA Licensed Clinical Lab)	Negative

Miscellaneous Tests

TEST	RESULTS
Inducible expression of CD 62E (E-Selectin)	> 90% positive by immunofluorescence
Cytoplasmic VWF / Factor VIII	> 95% positive by immunofluorescence
Cytoplasmic uptake of Di-I-Ac-LDL	> 95% positive by immunofluorescence

Additional Information

The functional test of ACBRI 539 for response to recombinant human IL-1ß stimulation in a human PMN Adherence Assay: recombinant human IL-1ß stimulation @ 15 CPD in vitro in CSC Complete Serum-Free Medium (SF-4Z0-500).

Citations
PMID	Title	Author	Year
21253500	The Traditional Japenese Formula Keishibukuryogan Inhibits the Production of Inflammatory Cytokines by Dermal Endothelial Cells	Yoko Yoshihisa	2010
17530642	Comprehensive investigation of disease-specific short peptides in sera from patients with systemic sclerosis: Complement C3f-des-arginine, detected predominantly in systemic sclerosis sera, enhances proliferation of vascular endothelial cells	Yang Xiang	2007
12388718	Interleukin-8 and Growth-Regulated Oncogene Alpha Mediate Angiogenesis in Kaposi's Sarcoma	Brian R. Lane	2002

ACBRI is a nonprofit research institute providing research support to biomedical and cell biology institutions/agencies/organizations. CSC is the authorized agent for distribution of ACBRI research material. Funds collected by CSC for ACBRI products are transferred to ACBRI to cover acquisition and research support costs.

IRB acquisition requirements prohibit involvement of ACBRI research material in any profit-making enterprise, and Cell Systems Corporation does not profit from handling or delivery of ACBRI products. There are specific restrictions on the employment of ACBRI cells. ACBRI cells are for research purposes only and may not be transferred out of the direct control of the Recipient Institution/agency/organization. ACBRI cells may not be genetically altered in any way without prior written permission by ACBRI. Use of ACBRI products (as documented by placement of any order for product by an authorized representative constitutes knowledge, understanding and binding acceptance of these restrictions on behalf of the recipient organization/institution.

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