Human Hepatocyte Cells

Certificate #: ACBRI 3716

Introduction

Primary Human Hepatocyte Cells (ACBRI 3716) were initiated from normal human liver tissues by centrifugal counterflow elutriation following dispase digestion of tissue.

Cell Initiation

These cells were originated using CSC Complete Serum-Free Medium (SF-4Z0-500), are available at <12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~ 1.5 X 10⁶ cells. This vial will initiate a Passage 4 cell culture in a 75cm² flask.

These cells are available in cryopreserved vials as well as in 25cm² and 75cm² proliferating cell culture flasks.

We have other individual donor lots available. Please contact us for details.

Companion Products

Each vial or flask of cells is shipped to Customer with Bac-Off^® (antibiotic) and CultureBoost (animal derived growth factors) or CultureBoost-R (human recombinant growth factors) at no additional cost.

These cells are qualified for use with: CSC Complete Serum Free Medium (SF-4Z0-500) and CSC Complete Medium which includes 10% serum (4Z0-500); CSC Attachment Factor™ (4Z0-210); CSC Passage Reagent Group™ (4Z0-800) and CSC Cell Freezing Medium (4Z0-705).

Standard Tests

TEST	RESULTS
HIV Serologic Test (donor level HIV AB EIA)	Negative
HIV PCR TEST (frozen cell pool by CLIA Licensed Clinical Lab)	Negative
Test of frozen cells for Mycoplasma spp. (ATCC method by CLIA Licensed Clinical Lab)	Negative

Citations
PMID	Title	Author	Year
15120618	Expression and induction of CYP3As in human fetal hepatocytes.	Tamihide Matsunaga	2004
12551989	Reduction of Natural Adenovirus Tropism to the Liver by both Ablation of Fiber-Coxsakievirus and Adenovirus Receptor Interaction and Use of Replaceable Short Fiber	Takafumi Nakamura	2003
14679308	Integrin-linked kinase regulates vascular morphogenesis induced by vascular endothelial growth factor	Yayoi Kaneko	2003
12379708	Tumor Necrosis Factor Alpha-Induced Intereukin-8 Production via NF-kB and Phosphatidylinositol 3-Kinase/Akt Pathways Inhibits Cell Apoptosis in Human Hepatocytes	Yosuke Osawa	2002
11418646	TNF-alpha-Induced Sphingosine 1-Phosphate Inhibits Apoptosis Through a Phosphatidylinositol 3-Kinase/Akt Pathway in Human Hepatocytes	Yosuke Osawa	2001
10864670	A Cytoplasmic RNA Vector Derived from Nontransmissible Sendai Virus with Efficient Gene Transfer and Expression	Hai-Ou Li	2000

ACBRI is a nonprofit research institute providing research support to biomedical and cell biology institutions/agencies/organizations. CSC is the authorized agent for distribution of ACBRI research material. Funds collected by CSC for ACBRI products are transferred to ACBRI to cover acquisition and research support costs.

IRB acquisition requirements prohibit involvement of ACBRI research material in any profit-making enterprise, and Cell Systems Corporation does not profit from handling or delivery of ACBRI products. There are specific restrictions on the employment of ACBRI cells. ACBRI cells are for research purposes only and may not be transferred out of the direct control of the Recipient Institution/agency/organization. ACBRI cells may not be genetically altered in any way without prior written permission by ACBRI. Use of ACBRI products (as documented by placement of any order for product by an authorized representative constitutes knowledge, understanding and binding acceptance of these restrictions on behalf of the recipient organization/institution.

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