Human Cardiac Fibroblast Cells

Certificate #: ACBRI 5118

Introduction

Primary Human Cardiac Fibroblast Cells (ACBRI 5118) were initiated by centrifugal counterflow elutriation of dispase dissociated human cardiac muscle.

Cell Initiation

These cells were originated using CSC Complete Serum-Free Medium (SF-4Z0-500), are available at < 12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~ 1.5 X 10⁶ cells. This vial will initiate a Passage 4 cell culture in a 75cm² flask.

These cells are available in cryopreserved vials as well as in 25cm² and 75cm² proliferating cell culture flasks.

Companion Products

Each vial or flask of cells is shipped to Customer with Bac-Off^® (antibiotic) and CultureBoost (animal derived growth factors) or CultureBoost-R (human recombinant growth factors) at no additional cost.

These cells are qualified for use with: CSC Complete Serum Free Medium (SF-4Z0-500) and CSC Complete Medium which includes 10% serum (4Z0-500); CSC Attachment Factor™ (4Z0-210); CSC Passage Reagent Group™ (4Z0-800) and CSC Cell Freezing Medium (4Z0-705).

Standard Tests

TEST	RESULTS
HIV Serologic Test (donor level HIV AB EIA)	Negative
HIV PCR TEST (frozen cell pool by CLIA Licensed Clinical Lab)	Negative
Test of frozen cells for Mycoplasma spp. (ATCC method by CLIA Licensed Clinical Lab)	Negative

Miscellaneous Tests

TEST	RESULTS
Cytoplasmic Vimentin Intermediate Filaments > 95% positive by immunofluorescence	> 95% positive by immunofluorescence

Citations
PMID	Title	Author	Year
18195003	FOXO3A Regulates Peroxiredoxin III Expression in Human Cardiac Fibroblasts	Dan Sorescu	2008
16179589	NAD(P)H Oxidase 4 Mediates Transforming Growth Factor-beta 1- Induced Differentiation of Cardiac Fibroblasts Into Myofibroblasts	Dan Sorescu	2005

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IRB acquisition requirements prohibit involvement of ACBRI research material in any profit-making enterprise, and Cell Systems Corporation does not profit from handling or delivery of ACBRI products. There are specific restrictions on the employment of ACBRI cells. ACBRI cells are for research purposes only and may not be transferred out of the direct control of the Recipient Institution/agency/organization. ACBRI cells may not be genetically altered in any way without prior written permission by ACBRI. Use of ACBRI products (as documented by placement of any order for product by an authorized representative constitutes knowledge, understanding and binding acceptance of these restrictions on behalf of the recipient organization/institution.

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