The Serum-Free System With RocketFuel™

Certificate #: CSS-A102

The Serum-Free System. Complete cell growth and passage system including 2 ea Serum-Free CSC Medium, 1 ea 100mL Attachment Factor™, 2ea, 8mL RocketFuel™, 1 set 100mL Passage Reagent Group™ and 50 mL Cell Freezing Medium™.

Medium Component: Serum-Free Medium.  This medium becomes complete once activated with the included RocketFuel™ (1- 8mL vial), containing animal derived growth factors. SF-4Z0-500 is Certified and intended for experimental application. 

Growth Supplement Component: RocketFuel™ is the broad-spectrum growth supplement designed for use with serum-free medium. RocketFuel™ is included in various CSC Serum-Free medium kits.

PRG Component: Passage Reagent Group™ (PRG) is a matched set of CSC Certified™ reagents for releasing cells from culture for subculture or freezing. The PRG contains three parts: PRG-1 (EDTA -dPBS Solution), PRG-2 (Trypsin/EDTA -dPBS Solution) and PRG-3 (Trypsin Inhibitor-dPBS Solution). The chelating agent EDTA in PRG-1 prepares for PRG-2, which contains highly purified trypsin. PRG-3 inactivates the protease in PRG-2 and stabilizes the cell membranes. 

Cell Freezing Medium™ Component: CSC Cell Freezing Medium™ is a specialized medium, when used in conjunction with CSC Passage Reagent Group™, provides a beneficial environment for the freeze/thaw cycle of cell cultures, assisting in the minimization of cellular damage during the process. 

Attachment Factor™ Component: Attachment Factor™ is an extracellular matrix (ECM) product that promotes cell attachment to tissue culture surfaces and encourages correct polarity and cytoskeletal organization. Attachment Factor™ also contributes to correct in vitro growth factor and biological response modifier presentation. Use of Attachment Factor™ is critically important when cultures are to be initiated, grown, passaged, or used within the CSC Medium family.

CSC mediums and reagents are Sterile, made with WFI and all components are cGMP and ISO Compliant.

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