Human Aortic Endothelial Cells

Certificate #: ACBRI 375

Introduction

Primary Human Aortic Endothelial Cells (ACBRI 375) were initiated from normal human descending aorta (aortic arch to renal artery).

Cell Initiation

These cells were originated using CSC Complete Serum-Free Medium (SF-4Z0-500), and subsequently grown and passaged in CSC Complete Medium (4Z0-500). They are available at Passage 3 [< 12 cumulative population doublings] cryopreserved in CSC Cell Freezing Medium™ (4Z0-705). This vial will initiate a Passage 4 cell culture in a 75cm2 flask.

In addition to cryopreserved vials, these cells also are available in 25cm2 and 75cm2 proliferating cell culture flasks (US domestic market only).

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Companion Products

Each vial of cells is shipped to Customer with Bac-Off® (antibiotic) and CultureBoost™ (animal derived growth factors) or CultureBoost-R™ (human recombinant growth factors) at no additional cost.

These cells are qualified for use with: CSC Complete Serum Free Medium (SF-4Z0-500) and CSC Complete Medium which includes 10% serum (4Z0-500); CSC Attachment Factor™ (4Z0-210); CSC Passage Reagent Group™ (4Z0-800) and CSC Cell Freezing Medium™ (4Z0-705).

Standard Tests

TEST

RESULTS

HIV Serologic Test (donor level HIV AB EIA)

Negative

HIV PCR TEST (frozen cell pool by CLIA Licensed Clinical Lab)

Negative

Test of frozen cells for Mycoplasma spp. (ATCC method by CLIA Licensed Clinical Lab)

Negative

 

Miscellaneous Tests

TEST

RESULTS

Inducible expression of CD 62E (E-Selectin)

 > 90% positive by immunofluorescence

Cytoplasmic VWF / Factor VIII

 > 95% positive by immunofluorescence

Cytoplasmic uptake of Di-I-Ac-LDL

 > 95% positive by immunofluorescence

 

Additional Information

The functional test of ACBRI 375 for response to bacterial LPS stimulation in a human PMN adherence assay was performed at 15 CPD in vitro in CSC Serum-Free Medium.

E. coli LPS (Sigma), Neutralizing monoclonal antibodies to CD62E, CD49B and CD18 are CSC Standard reagents.

Human polymorphonuclear leukocytes (PMN) were freshly prepared from peripheral venous blood by elutriation.

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Citations

PMID Title Author Year

17203242

Gene expression profiles of endothelial progenitor cells by oligonucleotide microarray analysis

Souichi Furuhata

2006

ACBRI cells are available for in vitro research purposes only and may not be transferred out of the direct control of the recipient Institution/Agency/Organization. ACBRI cells may not be genetically altered in any way without prior written permission from Cell Systems Corporation. Use of ACBRI materials (evidenced by placement of any order for product) constitutes knowledge, understanding and binding acceptance of these restrictions on behalf of the recipient Institution/Agency/Organization. 

The Applied Cell Biology Research Institute (ACBRI) was created to further the knowledge of eukaryotic cell biology through laboratory research, publications and teaching.  It provided cells and cell culture products to other research entities - public and private. Cell Systems Corporation is the custodian of all ACBRI materials.

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