Human Adult Dermal Microvascular Endothelial Cells

Certificate #: ACBRI 539

Introduction

Primary Adult Human Dermal Microvascular Endothelial Cells (ACBRI 539) were initiated from normal human ventral abdominal skin tissue.

Cell Initiation

These cells were originated using CSC Complete Serum-Free Medium (SF-4Z0-500), and subsequently grown and passaged in CSC Complete Medium (4Z0-500). They are available at Passage 3 [< 12 cumulative population doublings] cryopreserved in CSC Cell Freezing Medium™ (4Z0-705). This vial will initiate a Passage 4 cell culture in a 75cm2 flask.

In addition to cryopreserved vials, these cells also are available in 25cm2 and 75cm2 proliferating cell culture flasks (US domestic market only).

Companion Products

Each vial of cells is shipped to Customer with Bac-Off® (antibiotic) and CultureBoost™ (animal derived growth factors) or CultureBoost-R™ (human recombinant growth factors) at no additional cost.

These cells are qualified for use with: CSC Complete Serum Free Medium (SF-4Z0-500) and CSC Complete Medium which includes 10% serum (4Z0-500); CSC Attachment Factor™ (4Z0-210); CSC Passage Reagent Group™ (4Z0-800) and CSC Cell Freezing Medium™ (4Z0-705).

Standard Tests

TEST

RESULTS

HIV Serologic Test (donor level HIV AB EIA)

Negative

HIV PCR TEST (frozen cell pool by CLIA Licensed Clinical Lab)

Negative

Test of frozen cells for Mycoplasma spp. (ATCC method by CLIA Licensed Clinical Lab)

Negative

 

Miscellaneous Tests

TEST

RESULTS

Inducible expression of CD 62E (E-Selectin)

 > 90% positive by immunofluorescence

Cytoplasmic VWF / Factor VIII

 > 95% positive by immunofluorescence

Cytoplasmic uptake of Di-I-Ac-LDL

 > 95% positive by immunofluorescence

 

Additional Information

The functional test of ACBRI 539 for response to recombinant human IL-1ß stimulation in a human PMN Adherence Assay: recombinant human IL-1ß stimulation @ 15 CPD in vitro in CSC Complete Serum-Free Medium (SF-4Z0-500).

Enlarge Photo

Citations

PMID Title Author Year

 

22420966

A Novel Peptide to Treat Oral Mucositis Blocks Endothelial and Epithelial Cell Apoptosis

Xiaoyan Wu

2012

21253500

The Traditional Japenese Formula Keishibukuryogan Inhibits the Production of Inflammatory Cytokines by Dermal Endothelial Cells

Yoko Yoshihisa

2010

17530642

Comprehensive investigation of disease-specific short peptides in sera from patients with systemic sclerosis: Complement C3f-des-arginine, detected predominantly in systemic sclerosis sera, enhances proliferation of vascular endothelial cells

Yang Xiang

2007

12388718

Interleukin-8 and Growth-Regulated Oncogene Alpha Mediate Angiogenesis in Kaposi's Sarcoma

Brian R. Lane

2002

ACBRI cells are available for in vitro research purposes only and may not be transferred out of the direct control of the recipient Institution/Agency/Organization. ACBRI cells may not be genetically altered in any way without prior written permission from Cell Systems Corporation. Use of ACBRI materials (evidenced by placement of any order for product) constitutes knowledge, understanding and binding acceptance of these restrictions on behalf of the recipient Institution/Agency/Organization. 

The Applied Cell Biology Research Institute (ACBRI) was created to further the knowledge of eukaryotic cell biology through laboratory research, publications and teaching.  It provided cells and cell culture products to other research entities - public and private. Cell Systems Corporation is the custodian of all ACBRI materials.

Please read our Terms and Conditions
   

Copyright © 1997-2016 Cell Systems Corporation. All Rights reserved